General Cell Based Assays
Eurofins Discoverx PathHunter Human LTB4R Total GPCR Internalization Cell-Based Assay Kit (U2OS)
The PathHunter Human LTB4R Stable Cell Line Total GPCR Internalization Assay (U2OS) contains a stable clonal cell line used to measure LTB4R (GPCR) internalization via early-endosome localization. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
Eurofins Discoverx PathHunter eXpress EDNRB CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0593E2CP0M)
The PathHunter EDNRB eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. endothelin-3) based activation of EDNRB GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged EDNRB and EA-tagged β-Arrestin-2. Activation of EDNRB stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter Human Heregulin beta 1 (ErbB2-ErbB3) Functional Kinase Cell-Based Assay Kit (U2OS)
The PathHunter Human Heregulin beta 1 (ErbB2-ErbB3) Stable Cell Line Functional Kinase Assay (U2OS) measures ErbB2-ErbB3 receptor tyrosine kinase activity via ligand-induced SH2 domain protein recruitment. This is a complete kit that includes the cell vials and all required reagents needed for cell culture and running the assay.
ETON BIOSCIENCE INC L-LACTATE ASSAY KIT 5ML
Lactate dehydrogenase converts lactate and NAD+ into pyruvate and NADH. The Lactate Assay Kit is based on the reduction of the terazolium salt INT in a NADH-coupled enzyme reaction to formazan, which exhibits an absorbance maximum at 490 nm.
Sino Biological Human IL-1RAcP / IL-1R3 Protein (Fc Tag), Endotoxin-Free 1mg
A DNA sequence encoding the human IL1RAP (NP_002173.1) (Met1-Glu359) was expressed with the Fc region of human IgG1 at the C-terminus.
Abcam Uricase Assay Kit (Fluorometric)
Uricase Assay Kit (Fluorometric)
The product is subject to the following: Abcam Restricted Use Statement
Eurofins Discoverx PathHunter eXpress PPYR1 (NPY4R) CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0233E2CP0M)
The PathHunter PPYR1 (NPY4R) eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. pancreatic polypeptide) based activation of PPYR1 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged PPYR1 and EA-tagged β-Arrestin-2. Activation of PPYR1 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress OXTR Activated GPCR Internalization Assay, 2-Plate Kit. (93-0715E3CP0M)
The PathHunter OXTR Activated GPCR internalization eXpress kit provides an easy to-use cell based assay to study drug candidates (small molecules or biologics) affecting GPCR activation and internalization. This kit uses EFC technology to measure ligand-induced internalization of OXTR via detection of localization to the early endosome. The included cell line overexpresses EA-tagged β-Arrestin-2 as well as a ProLink-tagged early endosome protein and untagged OXTR. Upon ligand-induced receptor activation, β-Arrestin-2 is recruited to the activated GPCR and the resulting OXTR-arrestin complex is internalized to the early endosome, leading to production of EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cayman Chemical Bilirubin Conjugate (sodium salt), Purity: ≥95%, CAS: 68683-34-1, 50 mg
A stable, water-soluble ditaurate derivative of bilirubin meant to mimic endogenous bilirubin glucuronide derivatives; exhibits anti-oxidant and antimutagenic effects
Eurofins Discoverx PathHunter eXpress GPR52 CHO-K1 β-Arrestin Orphan GPCR Assay, 10-Plate Kit. (93-0371E2ACP1L)
The PathHunter GPR52 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR52 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR52 and EA-tagged β-Arrestin-2. Activation of GPR52 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. This is an orphan receptor without a known ligand. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress MTNR1A CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0951E2CP2M)
The PathHunter MTNR1A eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. 2-iodomelatonin) based activation of MTNR1A GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged MTNR1A and EA-tagged β-Arrestin-2. Activation of MTNR1A stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Eurofins Discoverx PathHunter eXpress GPR23 CHO-K1 β-Arrestin Orphan GPCR Assay, 2-Plate Kit. (93-0336E2ACP1M)
The PathHunter GPR23 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand based activation of GPR23 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged GPR23 and EA-tagged β-Arrestin-2. Activation of GPR23 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Cell Biolabs Inc CytoSelect™ 48-Well Cell Contraction Assay Kit (Floating Matrix Model), 48 assays
Several culture models exist to study fibroblasts' ability of to reorganize and contract collagen matrices in vitro. In the floating matrix contraction model, a freshly polymerized collagen matrix containing cells is released from the culture dish and allowed to float in culture medium, and contraction occurs in the absence of external mechanical load and without appearance of stress fibers in the cells. The CytoSelect™ 48-Well Cell Contraction Assay Kit provides a simple system to assess cell contractivity in vitro and screen cell contraction mediators using the floating matrix model. The kit's proprietary Cell Contraction Plate eliminates the matrix releasing step of the conventional contraction assay, providing a faster, higher-throughput method to assess cell contraction. Each kit provides sufficient quantities to perform up to 48 assays in a 48-well plate.
Aat Bioquest Cell Meter™ JC-10 Mitochondrion Membrane Potential Assay Kit *Optimized for Microplate Assays* | 500 Tests
Although JC-1 is widely used in many labs, its poor water solubility makes it hard to use for some applications. Even at 1 µM concentration, JC-1 tends to precipitate in aqueous buffer. JC-10 has been developed to be a superior alternative to JC-1 where high dye concentration is desired. Compared to JC-1, our JC-10 has much better water solubility. JC-10 is capable of entering selectively into mitochondria, and changes reversibly its color from green to orange as membrane potentials increase. This property is due to the reversible formation of JC-10 aggregates upon membrane polarization that causes shifts in emitted light from 520 nm (i.e., emission of JC-10 monomeric form) to 570 nm (i.e., emission of J-aggregate). When excited at 490 nm, the color of JC-10 changes reversibly from green to greenish orange as the mitochondrial membrane becomes more polarized.